Fig. 2

a Pre-operative clinical images with acrylic stent in place to measure the PPD, CAL in the region of mesiobuccal [8(9) mm]. b Preoperative digital intra oral radiograph with intrabony defect in relation to mesial aspect of 26. c Simplified papilla preservation done in relation to mesial of 26 and flaps raised, Intra-operative clinical measurement of the two wall defect from the CEJ to the base of the defect in relation to mesiobuccal aspect of 26 = 5mm. d Root conditioning done with tetracycline in relation to 26. e Defect site grafted with B-ostIN HT (HA60 %:BTCP40 %). f Amniotic membrane placed over the grafted site in relation to mesial aspect of 26. g External vertical mattress suturing done with 4 − 0 vicryl sutures. h Immediate post-operative digital intra oral radiograph showing complete defect fill in relation to mesial aspect of 26. i Six months post-operative clinical images with acrylic stent in place to measure the PPD, CAL in the region of mesiobuccal [2(3) mm]. j Six months post-operative RVG in relation to mesial aspect of 26 showing complete defect fill