Fig. 6
From: In vivo bone regeneration assessment of offset and gradient melt electrowritten (MEW) PCL scaffolds
Microscope images of H&E stained tissue sections of MEW PCL scaffolds implanted in rat calvarial defects, a) 4 and b) 8 weeks (top and bottom panels respectively) after surgery. B: new bone; Black arrow: osteoblasts; BM: newly formed bone matrix; ST: Soft tissue; P: pore remaining implanted scaffold material; Blue arrow: new bone forming; Green arrow: osteocytes; Yellow arrow: osteocytes in lacuna; Asterisk: blood vessel; The black scale bar represents 200 μm. c) The percentage of newly formed bone for each group was calculated 4 and 8 weeks postoperatively using Fiji ImageJ software. The values expressed are the mean ± SD (n = 3). Statistically significant differences between the mean %bone for the treatment groups when compared to each other were determined by ANOVA (Significant: *, ^, ■, ♦, ▼, ● all p < 0.0001)