Ionic silver functionalized ovine forestomach matrix – a non-cytotoxic antimicrobial biomaterial for tissue regeneration applications

Table 1 Microbial Species and Culture Conditions

Species	ATCC #	Type	Culture Conditions
Species	ATCC #	Type	Media	Temp (°C)	Time (hours)
Acinetobacter baumannii	19606	Gram negative bacterium	TSB	35 ± 5	24–48
Vancomycin Resistant Enterococcus faecalis (VRE)	51575	Gram positive bacterium, drug resistant	VRE Broth	35 ± 5	24–48
Escherichia coli	8739	Gram negative bacterium	TSB	35 ± 5	24–48
Methicillin Resistant Staphylococcus aureus (MRSA)	33591	Gram positive bacterium, drug resistant	TSB	35 ± 5	24–48
Staphylococcus epidermidis (coagulase negative)	12228	Gram positive bacterium	TSB	35 ± 5	24–48
Streptococcus pyogenes (Group A, β-hemolytic)	12344	Gram positive bacterium	BHI	35 ± 5	24–48
Pseudomonas aeruginosa	27853	Gram negative bacterium	TSB	35 ± 5	24–48
Aspergillus brasiliensis (niger) ^a	16404	Mold	SDA	25 ± 2	48–96
Candida albicans	10231	Yeast	TSB	25 ± 2	48–96
Candida parapsilosis	22019	Yeast	SDB	25 ± 2	48–96
Candida glabrata	90030	Yeast	SDB	25 ± 2	48–96

Tryptic Soy Broth (TSB), Brain Heart Infusion (BHI). Sabouraud Dextrose Broth/Agar (SDB/A)
^aSpore inoculum prepared by PBS flood harvesting of plate culture

ISSN: 2055-7124