Fig. 3

Decreased apoptosis in organotypic cochlear cultures of P4 mice exposed to GM and DEX. A. TUNEL assay was conducted on explant cultures of P4 mice cochlea prepared from groups treated with PBS only (a), 0.3 mM GM only (b), and following pretreatment with DEX concentrations of 5 μg/mL (c), 100 μg/mL (d) prior to GM exposure. B. Apoptotic hair cells (green fluorescence) were counted from the cochlear explant cultures of P4 mice. Data are mean ± SEM from three independent tests in each dose of pre-treated DEX with 0.3 mM GM. p < 0.05 as compared with the GM-alone group. (p < 0.05* and p < 0.005**, 400x magnification.)