Fig. 3
From: Liposomes targeting the cancer cell-exposed receptor, claudin-4, for pancreatic cancer chemotherapy
a Intracellular drug-release behavior of DOX-C-SNPs, observed by confocal laser-scanning microscopy after a 24 h incubation, compared with that of free DOX. b In vitro cytotoxicity of DOX-C-SNPs, DOX-SNPs and free DOX towards KPC960 cells after incubation for 24 h. Data are presented as means ± SDs (n = 5). c Cellular uptake of DOX-C-SNPs. KPC960 cells were treated with DOX-C-SNPs, with or without CPE pretreatment. After 24 h, the fluorescence of Cy5.5 (red) was measured by confocal laser-scanning microscopy. Scale bar: 50 nm. Nuclei were counterstained with DAPI (blue). Representative flow cytometry data are presented in (d)