Fig. 3

Cytotoxicity on 4T1 cells with the treatment of Gel and G@Gel with or without NIR in vitro. A Cell viability of 4T1 cells with different treatments. B Live and dead staining of 4T1 cells with various treatments (Scale bar = 200 µm). C Immunofluorescence staining of CRT in 4T1 cells (Scale bar = 20 µm). DAPI and CRT was stained blue and red, respectively. D ATP and(E) HMGB1 content in supernatant of 4T1 cells after various treatment. Data are shown as mean ± SD (n = 3). The comparison of two groups was followed by unpaired Student’s t-test (two-tailed). The level of significance was defined as *p < 0.05, **p < 0.01