Fig. 6

Therapeutic efficacy of hMSC spheroids in hindlimb ischemia mouse model. A Representative images of hindlimb ischemia mouse model 0, 3, 7, 14, and 21 days after treatment. B Limb salvage ratio 21 days after treatment (n = 10 per group). C Representative images of H&E, Masson’s trichrome, and Picro Sirius 21 days after treatment. Scale bars = 100 μm. D Representative immunohistochemical images of CD31 and SM-α (green) showing micro-blood vessels (day 21). Scale bars indicate 100 μm. The relative amount of E CD31 and F α-SMA were measured by qRT-PCR (n = 6, *p < 0.05 versus all other group, **p < 0.05 versus all other group except anti-gravity group, ***p < 0.05 versus all other group except hanging drop group) and G western blot analysis