Fig. 5From: Subaqueous 3D stem cell spheroid levitation culture using anti-gravity bioreactor based on sound wave superpositionPhenotype changes of hMSC sphroids cultured in anti-gravity bioreactor at 12 h. Relative angiogenic mRNA expression levels of A VEGF, B IGF-1, and C ANGPT2 in hMSCs. Relative senescent mRNA expression levels of D p16 and E p21 in hMSCs. Relative proliferative mRNA expression level of F PCNA in hMSC. mRNA expression was evaluated by qRT-PCR (n = 6, * p < 0.05 versus all other group). G Representative images of immunostaining for PCNA (green) in hMSCs. Scale bars = 100 μmBack to article page