Skip to main content
Fig. 6 | Biomaterials Research

Fig. 6

From: Cardiac-targeted delivery of nuclear receptor RORα via ultrasound targeted microbubble destruction optimizes the benefits of regular dose of melatonin on sepsis-induced cardiomyopathy

Fig. 6

Nuclear receptor RORα enhanced the antioxidant effect of melatonin in sepsis-induced cardiomyopathy. (A) DHE and 4-HNE immunohistochemical staining in the heart Sect. 20 h after CLP surgery with or without RORα delivery and melatonin supplementation (scale bar of 100 μm). DHE intensity was quantified to reflect myocardial ROS levels (n = 7–10, ~ 40–59 views per group). (B) JC-1 staining of heart section (scale bar of 100 μm). The ratio of JC-1 aggregates (red) in healthy mitochondria and JC-1 monomers (green) in depolarized mitochondria was used to assess mitochondrial membrane potential (n = 5–7, ~ 22–34 views per group). (C) Western blots and quantification of Keap1, Nrf2, HO-1, SOD2, NOX2, and NOX4 expression in the sepsis heart with or without RORα delivery and melatonin supplementation (n = 4)

Back to article page