Fig. 3

Preparation and assessment of plasmid/CMBs. (A) Schematic diagram of the preparation of CMB particles. (B) Representative images of CMBs under the transmission electron microscope (scale bar of 400 nm). (C) Size distribution of the CMBs. (D) Potential distribution of the CMBs. (E) The binding percentage of different doses of DNA on CMBs (n = 3). (F) Representative images of plasmid/CMBs. Plasmid labeled by PI stain (red) was merged with the outline of CMB (bright) (scale bar of 50 μm). A local image of CMB with or without plasmid was indicated by the white asterisk (*) and pound sign (#) (scale bar of 500 nm)