Skip to main content
Fig. 2 | Biomaterials Research

Fig. 2

From: Cardiac-targeted delivery of nuclear receptor RORα via ultrasound targeted microbubble destruction optimizes the benefits of regular dose of melatonin on sepsis-induced cardiomyopathy

Fig. 2

RORα overexpression improved the effect of melatonin treatment on LPS-induced cardiomyocyte injury in vitro. (A) H9C2 cells treated with a plasmid containing the GFP gene 48 h later showing a transfection rate of more than 80% (scale bar of 100 μm). (B) Western blots and quantification of RORα expression in H9C2 cells transfected with vehicle or RORα plasmid for 48 h or treated with melatonin for 24 h (n = 4). (C) The effect of LDH leak from RORα-overexpressed H9C2 cells treated by LPS and melatonin (n = 8). (D) DCFH-DA staining was performed to assess ROS generation in RORα-overexpressed H9C2 cells treated by LPS and melatonin (n = 4, ~ 20 views per group, scale bar of 50 μm). (E) TUNEL staining to assess apoptosis index in RORα-overexpressed H9C2 cells treated by LPS and melatonin (n = 4, ~ 20 views per group, scale bar of 100 μm)

Back to article page