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Fig. 5 | Biomaterials Research

Fig. 5

From: Development of cell-laden multimodular Lego-like customizable endometrial tissue assembly for successful tissue regeneration

Fig. 5

Synthesis of individual endometrial tissue blocks encapsulating various types of endometrial cells labeled with fluorescent dyes. The fabricated biomaterials encapsulating various endometrial cellular components were injected into casting molds for three different block types (square, rectangle, and circular shape) and then polymerized at room temperature. Next, polymerized tissue blocks were effectively released from the casting molds by pushing them with a separator (A). Each embedded endometrial cellular component was labeled with GFP (green fluorescent protein) or mCherry (red fluorescent protein) to track their incorporation into the injured sites when they were transplanted in vivo. Three different types (square, rectangle, and circular shape) of tissue blocks encapsulating specific endometrial cellular components, such as endometrial stem cells (B), stromal cells (C), vessel cells (D), macrophages (E), and myometrial cells (F), were properly fabricated, and labeled cells were homogenously distributed throughout individual tissue blocks

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