Fig. 4

Various encapsulated endometrial cells are distributed homogeneously throughout the fabricated biomaterials. For analysis of the distribution patterns of the embedded cells within individual endometrial tissue blocks, samples were incubated in optimized culture medium for each cell type and then incubated in nuclear-specific dye DAPI solution. Each sample was observed using a fluorescence microscope with appropriate filters. Various embedded endometrial cellular components within individual tissue blocks, such as endometrial stem cells (A), stromal cells (B), vessel cells (C), macrophages (D), and myometrial cells (E), were labeled with DAPI (blue color). The labeled cells were homogeneously distributed throughout biomaterial-based individual tissue blocks