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Fig. 2 | Biomaterials Research

Fig. 2

From: Stabilization and improved functionality of three-dimensional perfusable microvascular networks in microfluidic devices under macromolecular crowding

Fig. 2

MMC stabilized MVNs in microfluidic devices. MVNs formed by GFP-expressing HUVECs cultured under control (no MMC) or crowded conditions (+ MMC) were continuously monitored for 10 days. (A) Representative pictures depicting fluorescent MVNs formed by GFP-expressing HUVECs at different time points. (B) Quantification of number of vascular junctions and (C) tubule length per field of view (FOV) using ImageJ angiogenesis analyzer plug-in. *, p < 0.05; **, p < 0.01; ***, p < 0.001; ****, p < 0.0001. n = 5 biological replicates. Scale bar = 100 μm

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