Fig. 4

The degradation and cell fluorescence tracer effects of n-butanol-inspired small aperture scaffolds in vivo were assessed. a Surgical procedures involving RSF-rhodamine with subcutaneous transplantation in rats were conducted, b with immediate implantation conditions at 0 months and degradation conditions at three and four months. c DAPI staining was applied at three and four months to show the rhodamine area distribution. d A schematic procedure for cell fluorescence tracer testing in nude mice for seven days was described, e with the area of DIL (red)-labelled chondrocytes (chon) and DIO (green)-labelled BMSCs implanted on the materials for seven days being estimated. f, g ImageJ was used to quantify the DIO, DIL, and Rhodamine areas. * represents p < 0.05, ** represents p < 0.01, *** represents p < 0.001, and **** represents p < 0.0001