Fig. 8
ASMP-AP discerns PTU-induced autophagy in zebrafish. A CLSM images from zebrafishes treated with PTU and stained with ASMP-AP (10 μM, λex = 405 nm, λem = 560–620 nm) and LysoTracker green (1.0 μM, λex = 488 nm, λem = 500–550 nm). B Representative images of inhibition of pigmentation with PTU treatment. C The fluorescence intensity was quantified form three independent ROIs in individual animals by ImageJ software. Statistical analyses were performed with One-way Anova. **: p < 0.01 and ****: p < 0.0001. Scale bar, 100 µm