Fig. 4
Lysosomal pH detection in HeLa cells with ASMP-AP (10 μM). A) CLSM images of cells incubated with ASMP-AP at 37 °C for 30 min in varied pH buffer. B) The plots of relative fluorescence intensities of panel A vs varied pH value. The intensities were quantified from three ROIs in each image. C) CLSM images of cells in Hank's balanced salt solution at different time nodes or in nutrient-rich medium. D) Analysis of the fluorescence intensity of panel B (n = 3, by ImageJ software). Statistical analyses were performed with One-way Anova. ****: p < 0.0001.) λex = 405 nm; λem = 560–620 nm. Scale bars, 10 μm