Fig. 6

Transcriptome analysis and the cell type analysis in control (GFP) or pseudovirus of SARS-CoV-2 Omicron variant infected in human LOs (n = 3). A Volcano plot of differentially expressed genes (DEGs) of PV-infected LO samples compared with control samples. Horizontal and vertical dashed blue lines indicate p-value < 0.05 and fold change = 4, respectively. Red dots indicate significant (p-value < 0.05) DEGs. B Top 25 genes of significantly up- or downregulated DEGs. C Heatmap of enrichment with lung-related cell types in PV-infected LOs by referring to the CellMarker database. D Lung cell-specific markers whole-stained using LOs. LOs were fixed and analyzed with various cell-type markers through immunofluorescence and visualized through confocal microscopy. Scale bar: 20 μm