Fig. 4
From: A novel DNA double-strand breaks biosensor based on fluorescence resonance energy transfer
Dose-dependent detection of the WT-DSBS in response to ionizing radiation (IR). (a, c) Dose-dependent CFP, FRET, and CFP/FRET ratio images of the WT-DSBS in HEK293T cells irradiated with 0.1, 0.5, 2, or 5 Gy, and then stabilized in the incubator for 30 min (a) or 20 h (c). The color scale indicates high (red) and low (blue) levels of DSBs (scale bar = 10 μm). (b, d) Dose-dependent DSBs level using the WT-DSBS in HEK293T cells irradiated with 0.1, 0.5, 2, or 5 Gy and then stabilized in the incubator for 30 min (c) or 20 h (d). All error bars represent SEM (*P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001)