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Fig. 6 | Biomaterials Research

Fig. 6

From: Drug-loaded microbubble delivery system to enhance PD-L1 blockade immunotherapy with remodeling immune microenvironment

Fig. 6

Increased PD-L1 expression and cellular uptake in tumor tissues after DTX stimulation. a Immunofluorescence staining images for PD-L1 expression of 4T1 tumor sections after various treatments. Blue, DAPI-labeled nucleus; green, FITC–conjugated anti-PD-L1 antibody-labeled PD-L1 (scale bar = 50 μm). b In vivo fluorescence images of 4T1 tumor dissected from mice after different treatments (αPD-L1, MBs + αPD-L1, RD@MBs + αPD-L1). c Ex vivo fluorescence images of 4T1 tumor dissected from mice after different treatments (αPD-L1, MBs + αPD-L1, RD@MBs + αPD-L1). d Corresponding MFI of ex vivo fluorescence images (n = 3). e FCM results of uptake of Cy5.5-labeled αPD-L1 on 4T1 tumor organ after different treatments. f Corresponding quantification of uptake of Cy5.5-labeled αPD-L1 (n = 3). g Frozen section images of distribution of Cy5.5-labeled αPD-L1 on 4T1 tumor after different treatments. Blue, DAPI-labeled nucleus; green, Cy5.5-labeled αPD-L1; red, anti-CD31 antibody labeled blood vessels (scale bar = 200 μm). Data are expressed as mean ± SD. Statistical significances were calculated via Student’s t test, *p < 0.05, **p < 0.01 and ***p < 0.001

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