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Fig. 1 | Biomaterials Research

Fig. 1

From: Drug-loaded microbubble delivery system to enhance PD-L1 blockade immunotherapy with remodeling immune microenvironment

Fig. 1

Increased PD-L1 expression and cellular uptake in tumor cells after DTX stimulation. a-b FCM results of the PD-L1 expression on 4T1 cells after various treatments (PBS, R@MBs, D@MBs, RD@MBs) and quantification of the relative expression. c-d Western blot analysis of the relative expression of PD-L1 on tumor cells. e Representative CLSM images of PD-L1 immunofluorescence staining of 4T1 cells. Blue, DAPI-labeled nucleus; green, FITC–conjugated anti-PD-L1 antibody-labeled PD-L1 (scale bar = 50 μm). f-g FCM results of cellular uptake of Cy5.5-labeled αPD-L1 after PBS and RD@MBs treatments and the corresponding quantitative analysis of MFI. h CLSM images of cellular uptake of Cy5.5-labeled αPD-L1. Blue, DAPI-labeled nucleus; green, Cy 5.5-labeled αPD-L1 (scale bar = 50 μm). Data are expressed as mean ± SD (n = 3). Statistical significances were calculated via Student’s t test, *p < 0.05, **p < 0.01 and ***p < 0.001

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