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Fig. 3 | Biomaterials Research

Fig. 3

From: Hierarchical porous ECM scaffolds incorporating GDF-5 fabricated by cryogenic 3D printing to promote articular cartilage regeneration

Fig. 3

Biocompatibility and chondrogenic differentiation analysis of pure ECM scaffolds. A Live/dead staining (green: live cells, red: dead cells) of BMSCs on the pure ECM scaffold; B Percentage of live cells on scaffolds (n = 3); C CCK-8 assay results of BMSCs cultured on ECM scaffolds for 1 day, 4 days, and 7 days (n = 4); D Morphology of BMSCs on the pure ECM scaffold (red: F-actin, blue: nucleus); E Expression of SOX 9, ACAN and Col 2A1 of the SMSCs on 5, 7 and 9% ECM scaffolds (n = 3). Statistical analysis: *p < 0.05, **p < 0.01, ***p < 0.001

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