Fig. 5

Directional distributions of neurites of primarily cultured embryonic (E18) rat hippocampal neurons on a 1.0 wt% nFHs drop coated, b 0.1 wt% nFHs bar coated, c 1.0 wt% nFHs bar coated glass surface. The direction of nFH on a glass substrate is set as 0°. Plots of d full-width at half-maximum (FWHM) of neurite directional distribution curves, e maximum neurite length, f neurite straightness, and g number of neurites sprout from each neuronal cell body as a function of days in vitro (DIV) of primary culture, depending on the surface modification type (PLL, 1.0wt% 0.1wt% bar-coated, 1.0wt% drop-coated nFHs). Individual groups were compared using Student’s t-test. ***, P ≤ 0.001; **, P ≤ 0.01; *, P ≤ 0.05, NS = non-significant. Representative immunofluorescence microscopic images of neuronal cells on h 1.0 wt% nFHs drop-coated, i 0.1 wt% nFHs bar-coated, j 1.0 wt% nFHs bar-coated glass surface at 5 DIV. Blue: nucleus, Red: tau, Green: actin