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Fig. 2 | Biomaterials Research

Fig. 2

From: A simple method to isolate structurally and chemically intact brain vascular basement membrane for neural regeneration following traumatic brain injury

Fig. 2

Characteristics of the brain-VBM structure. A Compared with normal brain tissue (A. 1–3), the structure of brain-VBM was loose. Hematoxylin and toluidine blue staining indicated that the cellular components of brain-VBM were completely removed. TEM revealed that brain-VBM was composed of interlinked hollow basement membrane tubes (L: longitudinal view; T: transverse view). B-D Light microscopy and SEM showed that the porosity of brain-VBM was significantly increased after crosslinking, with an intersecting branch-like structure (E shows the distribution of different calibers of VBM). The 3D reconstruction (F) of the confocal scanning results (see video 1) of laminin-stained brain-VBM samples shows multiple views of the structure and morphology of brain-VBMs. The depth scale in F shows different colors according to the z-positions. Statistical significance was set at P < 0.05; *P < 0.05

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Biomaterials Research

ISSN: 2055-7124

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