Fig. 1

Preparation and crosslinking of brain-VBM: A Schematic showing the design of this study, including brain-VBM preparation and processing, cell compatibility testing, and repair of the animal cortex after injury. A and B Dynamic display of the process of preparing brain-VBM of the entire brain (arrow indicates the thicker tissue, but not the remaining brain parenchymal tissue). The time required for preparing VBM from the central nervous system of different sizes and tissue sources varied. C-E show significant improvement in the mechanical properties of the brain-VBM after genipin crosslinking: Brain-VBM had significant shape memory characteristics. The concentration of the crosslinking agent affected the brain-VBM volume recovery rate (C) and time required for shape recovery (F). Statistical significance was set at P < 0.05; *P < 0.05. # indicates nonsignificance