Fig. 7

Immunofluorescence staining of different groups in the full-thickness infected wound. a Immunofluorescence staining of TNF-α (red) and nuclei (blue) on day 10 in the full-thickness infected wound. b Immunofluorescence staining of VEGF (red) and nuclei (blue) on day 10 in the full-thickness infected wound. c Double immunofluorescence staining of CD31 (red) and α-smooth muscle actin (α-SMA, green). CD31+ structures (red) were surrounded by α-SMA positive cells (green), implying vascular ducts. d Double immunofluorescence staining of collagen I (Col-I, green) and fibroblast marker vimentin (red). e Quantification of relative TNF-α expression. f Quantification of relative VEGF expression. g Quantification of CD31 labeled structures. h Quantification of relative collagen expression. n = 3, ns represents no significant difference, *P < 0.05, **P < 0.01, ***P < 0.001. Scale bar: 50 μm