Fig. 6

EX-Netrin1 improved functional recovery and reduced histopathological injury in SCI rats. (A) MRI was used to detect spinal cord injury in rats after 28 days. The red dotted box indicated the site of spinal cord injury. (B) BBB score was used to evaluate the functional recovery of hind limbs in rats. (C) After 28 days, spinal cord tissue was collected and ELISA was performed to detect the contents of TNF-α, IL-1β and IL-6 in spinal cord. (D) HE and Nissl staining of spinal cord tissues. Red arrows indicated Nissl bodies. Scale bar: 50 μm. (E-F) Representative images showing neurofilaments (NFs, green) and glial fibrillary acidic protein (GFAP, red) staining of spinal cord tissues, and (F) the density ratios of NFs. (Error bars showed means ± SD; n = 8. *P < 0.05, **P < 0.01, ***P < 0.001, vs. Sham group; ^#P < 0.05, ^##P < 0.01, ^###P < 0.001, vs. Model+PBS group; ^P < 0.05, ^^P < 0.01, ^^^P < 0.001, vs. Model+EX-MSCs group; ^$P < 0.05, ^$$P < 0.01, ^$$$P < 0.001, vs. Model+EX-NC group)