Fig. 5

The function of EX-netrin1 was attenuated by the inhibition of Unc5b but could be reversed by PI3K activation. (A) Western blot showed that Unc5b was successfully inhibited by small interfering RNA (si-Unc5b). (B) Western blot suggested that transfection of si-Unc5b significantly mitigated the promoting effect of EX-Netrin1 on Unc5b expression in PC12 cells. (C) ELISA was performed to detect the contents of TNF-α , IL-1β and IL-6 in the cell supernatant. (D-E) Fluorescence microscope was used to take representative images of neuronal cells and measure axonal length. The white solid line represents the axon length. Scale bar: 20 μm. (F-G) Detection of pyroptosis-related proteins by western blot. (H-I) The PI3K/Akt/mTOR pathway-related proteins were detected by western blot. (EX-MSCs group: LPS+EX-MSCs group; EX-NC group: LPS+EX-NC group; EX-Netrin1 group: LPS+EX-Netrin1 group; si-Unc5b group: LPS+EX-Netrin1+si-Unc5b group; 740 Y-P group: LPS+EX-Netrin1+si-Unc5b+740 Y-P group. Error bars showed means ± SD; n = 3; ~~P < 0.01, vs. Vehicle group; *P < 0.05, **P < 0.01, ***P < 0.001, vs. EX-MSCs group; ^#P < 0.05, ^##P < 0.01, ^###P < 0.001, vs. EX-NC group; ^P < 0.05, ^^P < 0.01, ^^^P < 0.001, vs. EX-Netrin1 group; ^$P < 0.05, ^$$P < 0.01, ^$$$P < 0.001, vs. si-Unc5b group)