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Fig. 3 | Biomaterials Research

Fig. 3

From: Engineered exosomes enriched in netrin-1 modRNA promote axonal growth in spinal cord injury by attenuating inflammation and pyroptosis

Fig. 3

EX-Netrin1 alleviated LPS-induced inflammation and pyroptosis of PC12 cells, promoted nerve recovery, and upregulated the PI3K/Akt/mTOR pathway. (A) ELISA was performed to detect the contents of TNF-α , IL-1β and IL-6 in the cell supernatant. (B-C) Fluorescence microscope was used to take representative images of neuronal cells and measure axonal length. Actin was stained red and nuclei were stained blue with DAPI. Scale bar: 20 μm. (D-E) Detection of pyroptosis-related proteins by western blot. (F-G) The PI3K/Akt/mTOR pathway-related proteins were detected by western blot. (Error bars showed means ± SD; n = 3; *P < 0.05, **P < 0.01, ***P < 0.001, vs. Control group; #P < 0.05, ##P < 0.01, ###P < 0.001, vs. LPS+EX-MSCs group; ^P < 0.05, ^^P < 0.01, ^^^P < 0.001, vs. LPS+EX-NC group; $P < 0.05, $$P < 0.01, $$$P < 0.001, vs. LPS group)

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