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Fig. 1 | Biomaterials Research

Fig. 1

From: Engineered exosomes enriched in netrin-1 modRNA promote axonal growth in spinal cord injury by attenuating inflammation and pyroptosis

Fig. 1

Efficiency and kinetics of modRNA transfection in MSCs. (A–D) Transfection efficiency and the expression kinetics of modGFP in MSCs. (A) Representative images depicting GFP signal in MSCs at 4, 8, 16, 24, and 48 h post-transfection. Scale bar = 100 μm. (B-C) Flow cytometry analysis of transfection efficiency at 4, 8, 16, 24, and 48 h post-transfection. (D) Flow cytometry analysis of mean fluorescence signal intensity at 4, 8, 16, 24, and 48 h post-transfection. (E-G) Expression levels of (E) netrin-1 mRNA and (F–G) netrin-1 protein at 24 h post-transfection. (H) Kinetics of cumulative netrin-1 protein concentrations periodically monitored for several days following transfection of modNetrin-1 in MSCs. (I) Research flow for in vitro experiments. First, the modRNA was synthesized and transfected into MSCs. Then the engineered exosomes rich in Netrin-1 modRNA were isolated. Finally, observed the therapeutic effect of exosomes on LPS induced PC12 cells. (Error bars showed means ± SD; n = 3; *p < 0.05, **p < 0.01, ***p < 0.001)

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Biomaterials Research

ISSN: 2055-7124

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