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Fig. 1 | Biomaterials Research

Fig. 1

From: Confocal imaging of biomarkers at a single-cell resolution: quantifying 'living' in 3D-printable engineered living material based on Pluronic F-127 and yeast Saccharomyces cerevisiae

Fig. 1

Preparation of the hydrogel plugs. The 25% w/w F127-DMA was mixed with yeast cells and photoinitiator at 4 °C. The mixture was cast into the mold, incubated at 0 °C/30 min, incubated at 25 °C/30 min, cross-linked with 365 nm/120 s, and incubated at 25 °C/30 min. The hydrogel plug was taken from the mold and incubated at 30 °C/16 h in deionized water or chemically defined yeast medium, after which it was cut in half, and its inner cross-section imaged

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