Fig. 6

Promotion of muscle regeneration in the mouse model of VML by SCOS/MT constructs transplatation. a Schematic illustration of muscle repair assisted by SCOS/MT constructs after VML. b Macroscopic view of the transplanted sites treated with control, DDFS/MT, SCOS, and SCOS/MT after 8 weeks. c-d Representative H&E and Masson trichrome stained images of muscle tissues with sham or VML injury in quadriceps femoris muscles 8 weeks following transplantation. e Immunofluorescence staining of MyHC. f Immunohistochemical staining of AchR. g Immunofluorescence staining of α-SMA. h The quantitative results of de novo muscle formation according to MyHC staining. i-j The quantitative results of AchR-positive area and α-SMA-positive vessels, respectively (n = 5–6, mean ± SD). Scale bar: 100 μm. **** p < 0.0001, *** p < 0.001, ** p < 0.01, * p < 0.05, by One-way ANOVA test