Fig. 5
From: Self-curling 3D oriented scaffolds from fish scales for skeletal muscle regeneration
Enhanced myogenic differentiation in C2C12 cultured on DDFS and SCOS. a Immunofluorescence staining of C2C12 myotubes cultured on the cell culture plate (control) or DDFSs for myogenic marker myogenin (MYOG). b Quantitative analysis of MYOG-positive cells per field (n = 6, mean ± SD). c Myosin heavy chain (MyHC) immunostaining of C2C12 myotubes cultured on the cell culture plate (control) or DDFSs (scale bar: 100 μm). d Fusion index analysis based on MyHC staining (n = 6, mean ± SD). e-h Comparison of mRNA levels of myogenic markers including MyoG, MyoD1, Myf5 and MyHC by RT-qPCR analysis (n = 9, mean ± SD). i Western blot assay of protein levels of MyHC, MyoD1 and MyoG. j-l Quantitative analysis of Western blot results (n = 2, mean ± SD). Scale bar: 100 μm. **** p < 0.0001, *** p < 0.001, ** p < 0.01, * p < 0.05, by One-way ANOVA or two-tailed Student’s t test