Fig. 5

PTK7-GEMs internalization and trafficking in cells. Images generated using confocal microscopy revealing the co-localization of PTK7-GEMs-cy5 (red) with markers of endocytosis dextran (A), choleratoxin (B), transferrin (C) labeled with Alexa Fluor 488 respectively. D Pearson’s correlation coefficient analysis of PTK7-GEMs-cy5 and endocytosis marker co-localization in 5637 cells using Image J. Confocal microscopy images revealing the co-localization of PTK7-GEMs-cy5 (red) with EIPA (E) (inhibitor of macropinocytosis), Filipin (F) (inhibitor of the caveolae pathway), and Chlorpromazine (G) (inhibitor of the clathrin pathway) (green). DAPI was used to counterstain the nuclei (blue). Scale bar, 10 μm. The means ± standard deviation (n = 3 per group) is indicated using error bars. Each replicate was from a single biological experiment and 10 independent fields of view were chosen from quantification. *P < 0.05; NS = not significant