Fig. 2

Serum stability and cell binding ability of PTK7-GEMs. Serum stability analysis of PTK7-GEMs using 12% polyacrylamide gel electrophoresis (PAGE). A PTK7-GEMs were incubated for 0, 1, 2, 4, 8, 12, 16, and 24 h 10% FBS-supplemented or 10% NMS-supplemented RPMI-1640 medium, respectively. B The abscissa indicates that the same sample of PTK7-GEMs was incubated in 10% NMS (red) and 10% FBS (blue) for 0, 1, 2, 4, 8, 12, 16, and 24 h respectively, and the ordinate indicates the respective degradation percentage of the samples. The binding ability of PTK7-GEMs to PTK7 overexpressing cells at 4 °C, as determined using flow cytometry. C, D The binding ability of various FITC-labeled samples to 5637 cells and SV-HUC-1 cells