Fig. 4From: Biomimetic hydrogel blanket for conserving and recovering intrinsic cell propertiesConserving and recovery effects of intrinsic properties by DCP culture with the hydrogel blanket. Representative cell shape (A) and cell morphology (B) of relatively young to old chondrocytes in passage 2 (P2), passage 4 (P4), and passage 9 (P9) cultured in NC, NP, HC, and HP environments. (C) Col2 expression immunofluorescence staining confocal microscopy images of chondrocytes cultured in each environment. Cell shape occupancy rates according to passage and culture environment of NC (D), NP (E), HC (F), and HP (G). Results of comparative analysis of the amount of GAG/DNA after culturing cells of P2 (H), P4 (I), and P9 (J) in each environment of NC, NP, HC, and HP. PCR analysis results of SOX9 (K), TCF-β (L), HIF-1α (M), Col2/Col1 (N) expression of P2, P4, and P9 passage cells cultured in NC, NP, HC, and HP environments. Each graph of (H to N) represents the mean, standard deviation, and raw data. ANOVA analysis and Tukey’s post-hoc test were performed for statistical significance verification. N.S. (not significant), *P < 0.05, **P < 0.01, ***P < 0.001Back to article page