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Fig. 2 | Biomaterials Research

Fig. 2

From: Biomimetic hydrogel blanket for conserving and recovering intrinsic cell properties

Fig. 2

Changes in cell morphology according to the culture environment and numerical comparison of cell morphological changes using the classification criteria. (A) Cell culture conditions and schematic diagram: normal control (NC); a typical cell culture environment at 20% oxygen, 5% CO2, 95% or higher humidity, and 37℃. normal pressing (NP); hydrogel blanket applied in the same cultural environment as NC. hypoxia control (HC); hypoxic condition by artificially lowering the oxygen concentration to 1%. hypoxia pressing (HP); Artificially lowered oxygen concentration to 1% and additionally applied hydrogel blanket. (B) Phase-contrast microscopy images of chondrocytes cultured for 2 days in each environment of NC, NP, HC, and HP. Relative occupancy rate (C) according to the classification criteria of the cell shape (D). The representative morphology of shape 1 according to the cell shape classification criteria (E) and the proportion of shape 1 in each culture environment (F). The representative morphology of shape 2 according to the cell shape classification criteria (G) and the proportion of shape 2 in each culture environment (H). The representative morphology of shape 3 according to the cell shape classification criteria (I) and the proportion of shape 3 in each culture environment (J). In F-I, each graph represents the mean and standard deviation, and ANOVA analysis and Tukey’s post-hoc test were performed for statistical significance. N.S. (not significant), **P < 0.01, ***P < 0.001

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