Fig. 2From: Biomimetic hydrogel blanket for conserving and recovering intrinsic cell propertiesChanges in cell morphology according to the culture environment and numerical comparison of cell morphological changes using the classification criteria. (A) Cell culture conditions and schematic diagram: normal control (NC); a typical cell culture environment at 20% oxygen, 5% CO2, 95% or higher humidity, and 37℃. normal pressing (NP); hydrogel blanket applied in the same cultural environment as NC. hypoxia control (HC); hypoxic condition by artificially lowering the oxygen concentration to 1%. hypoxia pressing (HP); Artificially lowered oxygen concentration to 1% and additionally applied hydrogel blanket. (B) Phase-contrast microscopy images of chondrocytes cultured for 2 days in each environment of NC, NP, HC, and HP. Relative occupancy rate (C) according to the classification criteria of the cell shape (D). The representative morphology of shape 1 according to the cell shape classification criteria (E) and the proportion of shape 1 in each culture environment (F). The representative morphology of shape 2 according to the cell shape classification criteria (G) and the proportion of shape 2 in each culture environment (H). The representative morphology of shape 3 according to the cell shape classification criteria (I) and the proportion of shape 3 in each culture environment (J). In F-I, each graph represents the mean and standard deviation, and ANOVA analysis and Tukey’s post-hoc test were performed for statistical significance. N.S. (not significant), **P < 0.01, ***P < 0.001Back to article page