Table 1 Primers used for qRT-PCR analysis
Gene name | Forward sequence (5′-3′) | Reverse sequence (5′-3′) |
|---|---|---|
U6 | CTCGCTTCGGCAGCACATA | CGAATTTGCGTGTCATCCT |
hsa-miR-21-5p | GCCGCGTAGCTTATCAGACT | CAGTGCAGGGTCCGAGGTAT |
hsa-miR-199a-3p | CGCCGACAGTAGTCTGCACAT | CAGTGCAGGGTCCGAGGTAT |
hsa-miR-16-5p | GCCGTAGCAGCACGTAAATA | CAGTGCAGGGTCCGAGGTAT |
hsa-let-7a-5p | CGCCGTGAGGTAGTAGGTTGT | CAGTGCAGGGTCCGAGGTAT |
hsa-miR-26a-5p | CCGCCGTTCAAGTAATCCAGG | CAGTGCAGGGTCCGAGGTAT |
Mus PTEN | GAAAGGGACGGACTGGTGTA | AGTGCCACGGGTCTGTAATC |
Mus PI3K | ACCTGGACTTAGAGTGTGCC | TCAGCAGTGTCTCGGAGTTT |
Homo ANG1 | GGACAGCAGGAAAACAGAGC | GCCCTTTGAAGTAGTGCCAC |
Homo VEGF | GGAGGAGGGCAGAATCATCA | CTTGGTGAGGTTTGATCCGC |
Homo Bax | AAGAAGCTGAGCGAGTGTCT | GTTCTGATCAGTTCCGGCAC |
Homo Bcl-2 | GCCTTCTTTGAGTTCGGTGG | GAAATCAAACAGAGGCCGCA |
Homo b-actin | CCCTGGAGAAGAGCTACGAG | CGTACAGGTCTTTGCGGATG |