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Fig. 2 | Biomaterials Research

Fig. 2

From: High yield engineered nanovesicles from ADSC with enriched miR-21-5p promote angiogenesis in adipose tissue regeneration

Fig. 2

Generation and characterization of ADSC-EVs and ADSC-NVs. A TEM images of ADSC-EVs and ADSC-NVs. Scale bar: 100 nm. B NTA analysis of ADSC-EVs and ADSC-NVs. The peak diameter of both ranged between 100 and 150 nm. C, D The yields of EVs and NVs from equivalent ADSCs were examined by particle number (C) and the protein amount (D) (n = 3 per group). E Protein expression of TSG101, CD81, and CD29 was assessed by western blot analysis for ADSC-EVs (EVs), ADSC-NVs (NVs), and ADSC. F-H The internalization of PKH26-labeled ADSC-EVs and ADSC-NVs in equivalent amount particles by HUVECs was evaluated at 1, 2, 3 and 4 h respectively. The internalization rate (F) was measured by calculating area ratio between ADSC-EVs/ ADSC-NVs and HUVECs (n = 3 per group). G, H Representative images of internalization of ADSC-EVs or ADSC-NVs at 1, 2, 3, 4 h. Scale bar: 100 μm. Red: ADSC-EVs/NVs, Green: HUVECs, blue: Nucleus of HUVECs. ns, no significant difference, ***p < 0.001, ****p < 0.0001

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