Fig. 6

Network signal propagation velocity in hiPSC-derived cortical neurons and axonal conduction velocity in DRG neurons. A) Network propagation velocity in hiPSC-derived cortical neurons cultured on SCAD devices. a) Whole-sample spike amplitude map obtained with complementary metal-oxide semiconductor–multielectrode array (CMOS–MEA). Seven neurons were identified using the magnitude of spike amplitude. b) Spike raster plot for each neuron identified in a). c) Signal propagation velocity between neurons from the network. It was calculated from the distance between neurons and the delay between the first spike of a network burst of each neuron, n = 4. B) Measurement of axonal conduction velocity of dorsal root ganglion (DRG) neurons cultured on SCAD devices. a) Whole-sample spike amplitude map. b) Example of the calculation of axonal conduction velocity for one single neuron. A pathway map, extracted axon traces, and a linear fit were used to calculate the velocity