Skip to main content
Fig. 4 | Biomaterials Research

Fig. 4

From: A functional neuron maturation device provides convenient application on microelectrode array for neural network measurement

Fig. 4

Analysis of the signal low-frequency components after compound administration or electrical stimulation. A) Frequency analysis of the signal from 2D cultured neurons and neurons cultured on SCAD devices treated with 4-aminopyridine (4-AP) administration (0.3, 3, and 30 µm). a) Local field potential (LFP) of a network burst (NB) before and after administration of 0.3, 3, and 30 µm 4-AP recorded with the same electrode. LFPs high-pass filtered at 1 Hz were recorded. b) Corresponding scalograms of the LFPs measured during the application of 4-AP. The scalograms are computed from the raw traces, not the high-pass filtered data. c) Changes in the signal low-frequency component (below 250 Hz) after 4-AP administration normalized to those of controls measured in 2D cultured neurons and neurons cultured on SCAD devices. Data were quantified using the average wavelet transform coefficient per pixel in the corresponding scalogram, n = 6 for 2D cultured neurons and n = 8 for neurons cultured on SCAD devices. Data were expressed by means ± standard errors. Statistical analyses were performed using ANOVA followed by post hoc Dunnet’s test, ** p < 0.01. B) Frequency analysis of neurons cultured on SCAD devices before and after theta burst stimulation (TBS). a) LFPs of an NB before and after TBS obtained using the same electrode. b) Scalograms of the LFPs measured before and after TBS. c) Changes in the signal low-frequency component (below 250 Hz) before and after TBS. Data were quantified using the average wavelet transform coefficient per pixel in the corresponding scalogram, n = 5. Data were expressed by means ± standard errors. Statistical analyses were performed using two-tailed paired Student’s t-test, ** p < 0.01

Back to article page