Fig. 3

Pharmacological properties of spontaneous firing activity. A Typical spontaneous firing patterns of human induced pluripotent stem cell (hiPSC)-derived cortical neurons cultured on SCAD devices after 5 weeks of in vitro culture (5 WIV) after administration of 4-aminopyridine (4-AP), pilocarpine, picrotoxin, and D-( −)-2-amino-5-phosphonopentanoic acid (AP-5) at the indicated concentrations. B Heat map of 10 parameters used to analyze the effects of each compound at different concentrations. Statistical analyses were performed using ANOVA followed by post hoc Dunnet’s test, * p < 0.05, ** p < 0.01. C Scatter plots of principal component analysis (PCA) using a 5-parameter set (total number of spikes, duration of network bursts (NBs), intermaximum frequency interval (IMFI), coefficient of variation (CV) of NB duration, and CV of the number of spikes in an NB) for detecting drug effects