Fig. 2

Spontaneous firing of hiPSC-derived cortical neurons cultured on SCAD devices. A) Spike detection rate (number of spikes/50 ms) and corresponding raster plots for all 16 electrodes. Measurements were performed during 10 min at 3, 4, and 5 weeks of in vitro culture (WIV) in neurons cultured on SCAD devices and in 2D cultured neurons. B) Comparison of 4 parameters, i.e., total number of spikes, numbers of network bursts (NBs), duration of NB, and number of spikes in an NB, between 2D cultured samples (n = 5) and SCAD cultured samples (n = 20) at 3, 4, and 5 WIV. Data were expressed by means + standard errors. Statistical analyses were performed using one-way analysis of variance (ANOVA) followed by post hoc Dunnet’s test, * p < 0.05, ** p < 0.01. The F value in ANOVA of each parameter is 17.13 for total number of spikes, 40.08 for numbers of NBs, 40.47 for duration of NB, and 16.83 for number of spikes in an NB. C) mRNA levels of typical proteins from 2D cultured neurons (2D, n = 2) and neurons cultured on SCAD device (SCAD, n = 4). Data were normalized by 2D neurons and expressed by means + standard errors. Statistical analyses were performed using two-tailed paired Student’s t-test, *p < 0.05, p < 0.1. a) mRNA levels of VGLUT2 and GLUR2 are significantly higher in SCAD samples than in 2D samples. NF160 and Synaptophysin also expressed higher (p < 0.1) in SCAD samples. b) mRNA level of Notch1 is significantly lower in SCAD samples than in 2D samples. Nestin and MASH1 also expressed lower (p < 0.1) in SCAD samples.