Fig. 8

Changes in the adhesion behaviors of HGEs cultured on the chemical surfaces. A GO enrichment analysis of the 650 types co-upregulated genes on the biological process level; B Number of remined adhered HGEs cultured on four groups of surfaces; C Ratio of exfoliated HGEs cultured on four groups of surfaces; D Representative SEM images of spreading morphology of HGEs cultured on four groups of surfaces; E The representative images and (F)semi-quantitative statistical analysis of HGEs migration on four groups of surfaces during a wound healing assay; G GO enrichment analysis of the 650 types co-upregulated genes on the cellular component level; H) RT-qPCR results and (I) representative western blot images results of the expression of focal adhesion and hemidesmosome related genes and proteins; J SIM immunofluorescence showed the FN adsorption together with HGEs cytoskeleton on four surfaces; ratio of exfoliated HGEs cultured on ppAA surface with and without FAK and mTOR inhibitors (K), ITGβ1 blocking antibodies (L) and FN neutralize antibodies (M). Significant differences between two groups with a p < 0.05 are presented as follows: a) versus ppAA group, b) versus ppAC group, c) versus ppME group, and d) versus control group. *: p < 0.05, **: p < 0.01, ***: p < 0.001