Fig. 1

A Transepithelial medical devices are widely used in clinical practice and the clinical demand of transepithelial integration is very high. B The transepithelial integration is mainly determined by the amount epithelial barrier structures, which occur at the interface-epithelium interface. C Experimental flow of the study. a) Surface coatings with designed chemistry were generated by plasma polymerization. b) Serum protein absorption profile on each surface was dissected by Liquid Chromatography-Tandem Mass Spectrometry and the adsorption amount of key protein, fibronectin, was detected by ELISA assay. c) The conformational change and exposal of functional protein domains of adsorbed fibronectin were detected by Molecular Dynamics Simulation and verified by immunofluorescence (IF). d) The activation of human gingival epithelial cell membrane integrins by adsorbed fibronectin was analyzed by molecular docking calculation, real-time quantitative polymerase chain reaction (RT-qPCR), Western blot (WB) and IF. e) The activation of epithelial barrier structure related pathway was investigated using RNA-sequencing (RNA-seq), RT-qPCR and WB. f) The formation of epithelial barrier structure and cell adhesion behavior were eventually evaluated using RNA-seq, RT-qPCR, WB, Structured Illumination Microscopy, scanning electron microscope (SEM) and cell shedding test