Fig. 1
From: PEG-mediated transduction of rAAV as a platform for spatially confined and efficient gene delivery
PEG-enhanced infection of rAAV-CMV::GFP vectors in vitro. a GFP expression in HeLa, HEK293T, K562 and PC12 cell lines transduced with untreated rAAV9-CMV::GFP (top) and rAAV9-CMV::GFP with 3.6% (wt/vol) PEG additive (bottom), respectively, at the MOI of 5 × 105 v·g/cell. For each cell line, the left column shows the fluorescence images, the middle column shows the bright field images, and the right column shows the overlay images, respectively. Scale bars, 100 μm. b Quantitative analysis of the transduction efficiency of untreated rAAV9-CMV::GFP and rAAV9-CMV::GFP with 3.6% PEG additive, respectively, in different cell lines at the MOI of 5 × 105 v·g/cell. n = 3 wells in each group. Data are represented as mean ± SD. c Transgene expression of rAAV9-CMV::GFP in HeLa cells with different concentrations of PEG additive. The MOI was kept at 5 × 105 v·g/cell, and the concentrations of PEG additive were 0.2, 0.8, 1.6, and 3.6% (wt/vol) from top to bottom, respectively. Columns from left to right: green fluorescence, bright field, and overlay images. Scale bar, 100 μm. d Dependence of the transduction efficiency of rAAV9-CMV::GFP in HeLa cells on the concentration of PEG additive. The MOI was kept at 5 × 105 v·g/cell. n = 3 wells in each group. Data are represented as mean ± SD