Fig. 1
From: CDH17 nanobodies facilitate rapid imaging of gastric cancer and efficient delivery of immunotoxin
Recapitulation of CDH17 expression in gastric cancer samples and isolation of CDH17 nanobodies. a CDH17 RNA expression (TPM, RNAseq) in gastric cancers and normal stomach controls. n = 408 (tumors) and 211(controls). b CDH17 protein expression assessed by IHC in gastric TMA samples. n = 79. Scale bars, 100 μm. c Score percentage (left) and positivity rate (right) of CDH17 expression analyzed from b. d CDH17 protein expression in cell lines determined with western blot. e CDH17 immunostaining in cell membrane of IM95 and MKN45 cell lines. f SDS-PAGE gel analysis of recombinant CDH17 domain 1-3. g Nanobody screening against CDH17 domain 1-3 with phage display technology. h Sequences alignment of isolated A1 and E8 nanobodies with highlighted CDR regions