Fig. 5

SIRPα-v Exos ameliorate demyelination after ICH. A Representative immunofluorescence images of MBP and SMI32 in the ipsilateral STR on the 35th day post-ICH (scale bar = 100 μm). B Quantification of the fluorescence intensities of MBP and SMI32 and the ratio of SMI32 to MBP intensity in the ipsilateral STR (n = 8/group). Data are normalized to the intensities of contralateral hemispheres. C Representative TEM images of myelin integrity in the ipsilateral STR on the 35th day post-ICH or after sham surgery (scale bar = 1000 nm). Red arrows indicate medullated fibers, and blued arrows indicate damaged medullated fibers. D Frequency histograms of all the quantified normally myelinated axons showing the distribution of axon diameter. E Scatter plots of the g-ratio versus axon diameter in the sham-operated group or all ICH groups on the 35th day. F The g-ratio of myelinated axons with respect to the axon diameter at 0.4-μm intervals in the sham-operated group or all ICH groups on the 35th day post-ICH (n = 5/group). G Schematic illustration of the position of the stimulating (Stim) and recording (Rec) electrodes for CAP measurements in the CC/EC. H Representative traces of the evoked CAPs in the CC (stimulus, 2 mA; 0.48 mm lateral to the stimulating electrode) on the 35th day post-ICH. I Quantification of the amplitude of the evoked CAPs of myelinated N1 fibers in response to the increase in stimulus strength (0.0 ~ 0.20 mA). J N1 amplitude in response to a 0.2 mA stimulus on the 35th day post-ICH (n = 6/group). * P < 0.05, ** P < 0.01, *** P < 0.001 vs. Sham group; # P < 0.05, ## P < 0.01 vs. Con group; ▲ P < 0.05, ▲▲ P < 0.01 vs. NC Exo group; ns, no significance. All the data are presented as the mean ± SD