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Fig. 2 | Biomaterials Research

Fig. 2

From: Modified exosomal SIRPα variants alleviate white matter injury after intracerebral hemorrhage via microglia/macrophages

Fig. 2

Identification of MSCs and exosomes. A Primary cultivation of MSCs. Spindle-shaped or irregular polygonal cells grew in clusters, relatively sparse between the clusters (scale bar = 100 µm). B The expression of protein markers on the surface of MSCs. The expression of MSC surface markers. MSCs are CD11b/CD34/CD45/CD29+/CD44+/CD90+ cells. C PCR product of SIRPα variant primers on agarose gel electrophoresis. Transfected MSCs stably express SIRPα variants. D Western blot assay of CD63, CD81, and Alix. Both empty exosomes (NC Exo) and SIRPα variant-modified exosomes (SIRPα-v Exo) expressed the three biomarkers. E Representative TEM images of NC Exos and SIRPα-v Exos. NC Exos and SIRPα-v Exos appeared as double-concave disc-shaped vesicles with different diameters, and low-density bright areas could be observed in the vesicles (scale bar = 100 nm). F Fitting curves of NTA analysis. Diameter distribution of NC Exos (diameter: 96.8 ± 5.7 nm) and SIRPα-v Exos (diameter: 100.7 ± 7.3 nm) (n = 5). G Representative in vivo fluorescence images of time-dependent biodistribution of DiR-labeled SIRPα-v Exos in mice (n = 7). H Analysis of blood cell parameters from SIRPα WT, CD47 antibody (anti-CD47), SIRPα variants (SIRPα-v), or SIRPα-v Exo-treated animals (n = 8). All the data are presented as the mean ± SD

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