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Fig. 5 | Biomaterials Research

Fig. 5

From: Contact osteogenesis by biodegradable 3D-printed poly(lactide-co-trimethylene carbonate)

Fig. 5

SEM micrographs analyzing the remarkable globular accretions of the cement line matrix, totally covering, and anchored to the surface of PLATMC templates. a General view of the globular layer secreted by seeded hBMSCs on the surface of 3D-printed PLATMC templates at 14 days. At higher magnifications, the surface is totally covered with globular (vesicular) layer in addition to layers of homogenous structural matrix on the top of the globular layer. b SEM micrographs of PCL and PLATMC samples, at 21 and 28 days after Alizarin red dye and mineralized ECM extraction, showing the persistent anchorage of globular accretions (1–2 µm diameter/each) to PLATMC surface, while no remaining matrix or cells were noticed on PCL. Not the cells/matrix anchored to the top of the globular accretions (Green arrowheads) and the connecting fibrillar collagen (ORANGE arrowheads)

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